PIIINP antibody (AA 34-272) (Biotin)

Catalog No. ABIN1173224

The Rabbit Polyclonal anti-PIIINP antibody has been validated for WB, IHC and ICC. It is suitable to detect PIIINP in samples from Human.

Quick Overview for PIIINP antibody (AA 34-272) (Biotin) (ABIN1173224)

Target: PIIINP (Procollagen III N-Terminal Propeptide (PIIINP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIIINP antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-PIIINP Antibody (Biotin)

Binding Specificity: AA 34-272

Purpose: Biotin-Linked Polyclonal Antibody to Procollagen III N-Terminal Propeptide (PIIINP)

Sequence: MGHHHHHHSG SEF- HLGQSYA DRDVWKPEPC QICVCDSGSV LCDDIICDDQ ELDCPNPEIP FGECCAVCPQ PPTAPTRPPN GQGPQGPKGD PGPPGIPGRN GDPGIPGQPG SPGSPGPPGI CESCPTGPQN YSPQYDSYDV KSGVAVGGLA GYPGPAGPPG PPGPPGTSGH PGSPGSPGYQ GPPGEPGQAG PSGPPGPPGA IGPSGPAGKD GESGRPGRPG ERGLPGPPGI KGPAGIPGFP GMKGHRGFDG RN

Specificity: The antibody is a rabbit polyclonal antibody raised against PIIINP. It has been selected for its ability to recognize PIIINP in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAA573Hu01Polyclonal Antibody to Procollagen III NTerminal Propeptide (PIIINP)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PIIINP

Target: PIIINP (Procollagen III N-Terminal Propeptide (PIIINP))

Alternative Name: Procollagen III N-Terminal Propeptide

Background: P3NP, N-Propeptide Of Type III Procollagen, Procollagen III Amino Terminal Propeptide