PIIINP antibody (AA 24-153)

Catalog No. ABIN1173222

Product Details anti-PIIINP Antibody

Target: PIIINP (Procollagen III N-Terminal Propeptide (PIIINP))

Binding Specificity: AA 24-153

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIIINP antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Polyclonal Antibody to Procollagen III N-Terminal Propeptide (PIIINP)

Specificity: The antibody is a rabbit polyclonal antibody raised against PIIINP. It has been selected for its ability to recognize PIIINP in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Procollagen III N-Terminal Propeptide (PIIINP) corresdonding to Two N-terminal Tags, His-tag and SUMO-tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PIIINP

Target: PIIINP (Procollagen III N-Terminal Propeptide (PIIINP))

Alternative Name: Procollagen III N-Terminal Propeptide (PIIINP Products)

Background: P3NP, N-Propeptide Of Type III Procollagen, Procollagen III Amino Terminal Propeptide