Serotonin antibody (FITC)

Catalog No. ABIN1173649

This Rabbit Polyclonal antibody specifically detects Serotonin in IHC, WB, ICC and IF. It exhibits reactivity toward Various Species.

Quick Overview for Serotonin antibody (FITC) (ABIN1173649)

Target: Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Reactivity: Various Species

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin antibody is conjugated to FITC

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Serotonin Antibody (FITC)

Purpose: FITC-Linked Polyclonal Antibody to 5-Hydroxytryptamine (5-HT)

Specificity: The antibody is a rabbit polyclonal antibody raised against 5-HT. It has been selected for its ability to recognize 5-HT in immunohistochemical staining and western blotting.

Cross-Reactivity: Various Species

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAA808Ge01Polyclonal Antibody to 5Hydroxytryptamine (5HT)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Serotonin

Target: Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Alternative Name: 5-Hydroxytryptamine

Target Type: Chemical

Background: 5HT, Serotonin, N-Methyl-Gamma