CD73 antibody (AA 29-550) (Biotin)

Catalog No. ABIN1174422

This anti-CD73 antibody is a Rabbit Polyclonal antibody detecting CD73 in WB, IHC and ICC. Suitable for Rat.

Quick Overview for CD73 antibody (AA 29-550) (Biotin) (ABIN1174422)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-CD73 Antibody (Biotin)

Binding Specificity: AA 29-550

Purpose: Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

Sequence: MGHHHHHHSG SEF-WE LTIMHTNDVH SRLEQTSDDS TKCLNASLCV GGVARLFTKV QQIRKEEPNV LLLDAGDQYQ GTIWFTVYKG LEVAHFMNLL GYDAMALGNH EFDNGVEGLI DPLLRNVKFP ILSANIKARG PLAPQISGLY LPYKVLSVGG EVVGIVGYTS KETPFLSNPG TNLVFEDEVT ALQPEVDKLK TLNVNKIIAL GHSGFEMDKL IAQKVRGVDV VVGGHTNTFL YTGNPPSKEV PAGKYPFIVT SDDGRKVPVV QAYAFGKYLG YLKVEFDDKG NVVTSYGNPI LLNSTIREDA AIKADINQWR IKLDNYSTQE LGRTIVYLNG SAQECRFREC NMGNLICDAM INNNLRHPDE MFWNHVSMCI VNGGGIRSPI DERNNGTITW ENLAAVLPFG GTFDLVQLKG STLKKAFEHS VHRYGQSTGE FLQVGGIHVV YDISRKPWDR VVQLKVLCTK CRVPIYEPLE MDKVYKVVLP SYLVNGGDGF QMIKDELLKH DSGDQDISVV SEYISKMKVI YPAVEGRIKF

Specificity: The antibody is a rabbit polyclonal antibody raised against CD73. It has been selected for its ability to recognize CD73 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAB250Ra01Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: Cluster Of Differentiation 73

Background: NT5E, NT5-E, E5NT, E5-NT, NTE, EN, ENT, 5'-Nucleotidase, Ecto

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process