Integrin beta 3 antibody (AA 135-377) (FITC)

Catalog No. ABIN1174442

The Rabbit Polyclonal anti-Integrin beta 3 antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect Integrin beta 3 in samples from Human.

Quick Overview for Integrin beta 3 antibody (AA 135-377) (FITC) (ABIN1174442)

Target: Integrin beta 3 (ITGB3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin beta 3 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Integrin beta 3 Antibody (FITC)

Binding Specificity: AA 135-377

Purpose: FITC-Linked Polyclonal Antibody to Integrin Beta 3 (ITGb3)

Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- DYPVDI YYLMDLSYSM KDDLWSIQNL GTKLATQMRK LTSNLRIGFG AFVDKPVSPY MYISPPEALE NPCYDMKTTC LPMFGYKHVL TLTDQVTRFN EEVKKQSVSR NRDAPEGGFD AIMQATVCDE KIGWRNDASH LLVFTTDAKT HIALDGRLAG IVQPNDGQCH VGSDNHYSAS TTMDYPSLGL MTEKLSQKNI NLIFAVTENV VNLYQNYSEL IPGTTVGVLS MDSSNVLQLI VDAYGKI

Specificity: The antibody is a rabbit polyclonal antibody raised against ITGb3. It has been selected for its ability to recognize ITGb3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant ITGb3 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against ITGb3 conjugated to fitc.

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: Integrin Beta 3

Background: CD61, ITG-B3, GP3A, GP3-A, GpIIIa, Platelet Membrane Glycoprotein IIIa

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex