APOB antibody (AA 3329-3494) (Biotin)

Catalog No. ABIN1175346

This anti-APOB antibody is a Rabbit Polyclonal antibody detecting APOB in WB, IHC and ICC. Suitable for Rat.

Quick Overview for APOB antibody (AA 3329-3494) (Biotin) (ABIN1175346)

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOB antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-APOB Antibody (Biotin)

Binding Specificity: AA 3329-3494

Purpose: Biotin-Linked Polyclonal Antibody to Apolipoprotein B (APOB)

Sequence: MGHHHHHHSG S-FL SSSSFVTDAL QYKLEGTSRL MRKKVLKLAT AVSLTNKFLK GSHDSTISLT KKNMEASVKT TANLHAPIFT MNFKQELNGN TKSKPTVSSS IELNYDFNSS KLHSAAKGGV DHKFSLESLT SYLSIESFTK GNIKGSFLSQ EYSGSVANEA NVYLNSKGTR SSVR

Specificity: The antibody is a rabbit polyclonal antibody raised against APOB. It has been selected for its ability to recognize APOB in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant APOB expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against APOB conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: Apolipoprotein B

Background: Apo-B, FLDB, Apo B-100, Apo B-48

Pathways: Lipid Metabolism