Integrin beta 7 Chain antibody

Catalog No. ABIN1176850

This Rat Monoclonal antibody specifically detects Integrin beta 7 Chain in BR and FACS. It exhibits reactivity toward Human and Mouse. It has been mentioned in 6+ publications

Quick Overview for Integrin beta 7 Chain antibody (ABIN1176850)

Target: Integrin beta 7 Chain

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Application: Blocking Reagent (BR), Flow Cytometry (FACS)

Clone: FIB27

Product Details

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: AKR/Cum mouse spontaneous T lymphoma cell line TK1

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Karecla, Green, Bowden, Coadwell, Kilshaw: "Identification of a binding site for integrin alphaEbeta7 in the N-terminal domain of E-cadherin." in: The Journal of biological chemistry, Vol. 271, Issue 48, pp. 30909-15, (1997) (PubMed).

Tidswell, Pachynski, Wu, Qiu, Dunham, Cochran, Briskin, Kilshaw, Lazarovits, Andrew, Butcher, Yednock, Erle: "Structure-function analysis of the integrin beta 7 subunit: identification of domains involved in adhesion to MAdCAM-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 3, pp. 1497-505, (1997) (PubMed).

Andrew, Berlin, Honda, Yoshino, Hamann, Holzmann, Kilshaw, Butcher: "Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 9, pp. 3847-61, (1994) (PubMed).

Lefrançois, Barrett, Havran, Puddington: "Developmental expression of the alpha IEL beta 7 integrin on T cell receptor gamma delta and T cell receptor alpha beta T cells." in: European journal of immunology, Vol. 24, Issue 3, pp. 635-40, (1994) (PubMed).

Roberts, Kilshaw: "The mucosal T cell integrin alpha M290 beta 7 recognizes a ligand on mucosal epithelial cell lines." in: European journal of immunology, Vol. 23, Issue 7, pp. 1630-5, (1993) (PubMed).

Kilshaw, Murant: "Expression and regulation of beta 7(beta p) integrins on mouse lymphocytes: relevance to the mucosal immune system." in: European journal of immunology, Vol. 21, Issue 10, pp. 2591-7, (1991) (PubMed).

Target Details

Target: Integrin beta 7 Chain

Background: The FIB27 antibody reacts with the integrin beta7 chain (also known as integrin betap), which is found in association with either of two alpha chains. alpha4beta7 (LPAM-1) is expressed on most mature lymphocytes and on small subsets of thymic and bone marrow cells, it interacts with several ligands, including VCAM-1, fibronectin, and MAdCAM-1. alphaIELbeta7 is found primarily on intestinal intraepithelial lymphocytes (IEL), and it may be involved in interactions between IEL and epithelia via recognition of E-cadherin. The FIB27 antibody blocks beta7 integrin-mediated adhesion to fibronectin, MAdCAM-1, and VCAM-1, and it cross-reacts with human integrin beta7.