CD20 antibody (Cytoplasmic) (APC-H7)

Catalog No. ABIN1176878

The Mouse Monoclonal anti-CD20 antibody has been validated for ICS. It is suitable to detect CD20 in samples from Human. There is 1 publication available.

Quick Overview for CD20 antibody (Cytoplasmic) (APC-H7) (ABIN1176878)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD20 antibody is conjugated to APC-H7

Application: Intracellular Staining (ICS)

Clone: H1

Product Details anti-CD20 Antibody (APC-H7)

Binding Specificity: Cytoplasmic

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human B lymphoma cell line

Isotype: IgG2a kappa

Application Details

Comment:

BALB/c

Sample Volume: 5 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with APC-H7 under optimum conditions, and unconjugated antibody and APC-H7 were removed.

References for anti-CD20 antibody (APC-H7) (ABIN1176878)

Cragg, Walshe, Ivanov, Glennie: "The biology of CD20 and its potential as a target for mAb therapy." in: Current directions in autoimmunity, Vol. 8, pp. 140-74, (2004) (PubMed).

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: CD20

Background: The H1 (FB1) antibody specificially binds to a cytoplasmic domain of CD20. CD20 is a 33-37-kDa four transmembrane phosphoprotein that is expressed by B lymphocytes from the pre-B stage and most malignant B cells and is lost during plasma cell differentiation. Low level CD20 expression is observed on a subset of normal circulating T lymphocytes, and CD20-positive T-cell lymphomas have been reported. The CD20 molecule is associated with membrane lipid raft domains, acts as a channel for calcium ions, and is involved in the regulation of B cell activation and survival. The cytoplasmic domain regions are serine and threonine rich and contain multiple phosphorylation consensus sequences.
Synonyms: MS4A1, membrane-spanning 4-domains subfamily A member 1, B1, Bp35, LEU-16