IFN alpha 2b antibody

Catalog No. ABIN1176895

This Mouse Monoclonal antibody specifically detects IFN alpha 2b in ICS and Neut. It exhibits reactivity toward Human. It has been mentioned in 6+ publications

Quick Overview for IFN alpha 2b antibody (ABIN1176895)

Target: IFN alpha 2b

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Intracellular Staining (ICS), Neutralization (Neut)

Clone: 7N4-1

Product Details anti-IFN alpha 2b Antibody

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IFN-alpha. Neutralization activity may be measured using MTT dye conversion in a IFN-alpha mediated EMC viral resistance assay using A549 as indicator cells.
50% Neutralization (ND50) at 0.3 - 3 µg/mL
>95% Neutralization at 10 - 50 myg/ml

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Human IFN-alpha2b

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Conjugated formats of this clone may be used for immunofluorescent staining, such as PE Mouse Anti-Human IFN-alpha[2b] (Cat. No. 560097) or Alexa Fluor® 647 Mouse Anti-Human IFN-alpha[2b] (Cat. No. 560088).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IFN alpha 2b antibody (ABIN1176895)

Pestka: "The human interferon alpha species and receptors." in: Biopolymers, Vol. 55, Issue 4, pp. 254-87, (2001) (PubMed).

Siegal, Kadowaki, Shodell, Fitzgerald-Bocarsly, Shah, Ho, Antonenko, Liu: "The nature of the principal type 1 interferon-producing cells in human blood." in: Science (New York, N.Y.), Vol. 284, Issue 5421, pp. 1835-7, (1999) (PubMed).

Dipaola, Smith, Ferencz-Biro, Liao, Testa: "Interferon-alpha 2 produced by normal human leukocytes is predominantly interferon-alpha 2b." in: Journal of interferon research, Vol. 14, Issue 6, pp. 325-32, (1995) (PubMed).

Lydon, Favre, Bove, Neyret, Benureau, Levine, Seelig, Nagabhushan, Trotta: "Immunochemical mapping of alpha-2 interferon." in: Biochemistry, Vol. 24, Issue 15, pp. 4131-41, (1985) (PubMed).

Pestka: "The human interferons--from protein purification and sequence to cloning and expression in bacteria: before, between, and beyond." in: Archives of biochemistry and biophysics, Vol. 221, Issue 1, pp. 1-37, (1983) (PubMed).

Green, Yeh, Overall: "Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons." in: Journal of clinical microbiology, Vol. 12, Issue 3, pp. 433-8, (1981) (PubMed).

Target Details for IFN alpha 2b

Target: IFN alpha 2b

Alternative Name: IFN-alpha 2b

Background: The 7N4-1 antibody reacts with human IFN-alpha2b and to a lesser extent with IFN-alpha7. It does not react with IFN-alpha1 nor IFN-alpha4. IFN-alpha2b is one of the three variants of IFN-alpha2 that have been isolated from human cell lines. IFN-alpha2b is the variant predominantly produced by human leukocytes. Human IFN-alpha2b belongs to the IFN-alpha class of proteins also known as leukocyte interferons. IFN-alpha comprises a family of related but distinct proteins with molecular weights ranging from 16-27 kDa with antiviral, antiproliferative and immunomodulatory activities. The IFN-alpha family is composed from as many as 14 different genes. The immunogen used to generate the 7N4-1 hybridoma was E. coli-expressed recombinant human IFN-alpha2b. This is a neutralizing antibody.
Synonyms: IFNa, IFNalpha