IRAK4 antibody (Alexa Fluor 647)

Catalog No. ABIN1176905

The Mouse Monoclonal anti-IRAK4 antibody has been validated for ICS. It is suitable to detect IRAK4 in samples from Human. There are 4+ publications available.

Quick Overview for IRAK4 antibody (Alexa Fluor 647) (ABIN1176905)

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IRAK4 antibody is conjugated to Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: L29-525

Product Details anti-IRAK4 Antibody (Alexa Fluor 647)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human IRAK4 Recombinant Protein

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells and cell lines (using BD™ Phosflow Lyse/Fix Buffer or BD Cytofix™ Fixation Buffer), but not human whole blood. BD™ Phosflow Perm Buffer II or Perm Buffer III may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IRAK4 antibody (Alexa Fluor 647) (ABIN1176905)

Suzuki, Saito: "IRAK-4--a shared NF-kappaB activator in innate and acquired immunity." in: Trends in immunology, Vol. 27, Issue 12, pp. 566-72, (2006) (PubMed).

Hatao, Muroi, Hiki, Ogawa, Mimura, Kaminishi, Tanamoto: "Prolonged Toll-like receptor stimulation leads to down-regulation of IRAK-4 protein." in: Journal of leukocyte biology, Vol. 76, Issue 4, pp. 904-8, (2004) (PubMed).

Lye, Mirtsos, Suzuki, Suzuki, Yeh: "The role of interleukin 1 receptor-associated kinase-4 (IRAK-4) kinase activity in IRAK-4-mediated signaling." in: The Journal of biological chemistry, Vol. 279, Issue 39, pp. 40653-8, (2004) (PubMed).

Li, Strelow, Fontana, Wesche: "IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 8, pp. 5567-72, (2002) (PubMed).

Target Details for IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Alternative Name: IRAK4

Background: Interleukin 1 (IL-1) is a proinflammatory cytokine that mediates the host inflammatory response. IL-1 exerts its biological effects through its type I receptor (IL-1RI), which is found on most cell types. When IL-1 binds to the IL-1RI, NF-kappaB (transcription factor) is activated and translocates to the nucleus where it induces gene expression. IL-1-receptor-associated kinase (IRAK) has been reported to co-immunoprecipitate with IL-1RI. The IRAK family is made up of four identified Pelle homologs that regulate the activation of NF-kappaB and mitogen-activated protein (MAP) kinase pathways. IRAK4 is a unique member of the IRAK family because it is the closest mammalian homolog to Pelle. Also, when overexpressed, its kinase activity allows it to activate both the NF-kappaB and mitogen-activated protein (MAP) kinase pathways, and block the IL-1-induced activation and modification of IRAK1. IRAK4 is also able to phosphorylate IRAK1, which suggests that IRAK4 affects the early signal transduction of Toll/IL-1 receptors before the involvement of IRAK1. The L29-525 monoclonal antibody recognizes human IRAK4, regardless of phosphorylation status. The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure). Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis.
Synonyms: IRAK-4, REN64, Q69FE3

Pathways: NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades