PKC alpha antibody (AA 270-427) (Alexa Fluor 488)

Catalog No. ABIN1176927

Cited in 4+ publications. The Alexa Fluor 488-conjugated Mouse Monoclonal anti-PKC alpha antibody (Clone 3-PKCalpha) (ABIN1176927) specifically detects PKC alpha in ICS. The antibody is reactive with Human, Mouse, Rat, Dog, Chicken and Frog samples.

Quick Overview for PKC alpha antibody (AA 270-427) (Alexa Fluor 488) (ABIN1176927)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC alpha antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Clone: 3-PKCalpha

Product Details anti-PKC alpha Antibody (Alexa Fluor 488)

Binding Specificity: AA 270-427

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PKCalpha aa. 270-427 Recombinant Protein

Isotype: IgG2b kappa

Application Details

Application Notes: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-PKC alpha antibody (Alexa Fluor 488) (ABIN1176927)

Braz, Gregory, Pathak, Zhao, Sahin, Klevitsky, Kimball, Lorenz, Nairn, Liggett, Bodi, Wang, Schwartz, Lakatta, DePaoli-Roach, Robbins, Hewett, Bibb, Westfall, Kranias, Molkentin: "PKC-alpha regulates cardiac contractility and propensity toward heart failure." in: Nature medicine, Vol. 10, Issue 3, pp. 248-54, (2004) (PubMed).

Parker, Murray-Rust: "PKC at a glance." in: Journal of cell science, Vol. 117, Issue Pt 2, pp. 131-2, (2003) (PubMed).

Zhang, Bontrager, Hemler: "Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta(1) integrins." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25005-13, (2001) (PubMed).

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, (1988) (PubMed).

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PKCa

Background: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. Conventional PKC (cPKC) subfamily members (a, b, and gamma isoforms) consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the the C2-like domains of novel PKC (nPKC) subfamily members (delta, epsilon, eta, and theta isoforms) are Ca2+-independent. The atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) lack the C2 domain and are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. PKCalpha regulates a wide variety of functions such as cellular growth, apoptosis, cardiomyocyte function, and brain cognitive functions. The 3/PKCalpha monoclonal antibody recognizes PKCalpha, regardless of phosphorylation status, and has been reported to crossreact with PKCbeta.
Synonyms: Protein Kinase C alpha, PKC-alpha, PKC-A, PKCA, PRKCA

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling