TWEAK antibody

Catalog No. ABIN1176948

This anti-TWEAK antibody is a Mouse Monoclonal antibody detecting TWEAK in FACS. Suitable for Human. This Primary Antibody has been cited in 5+ publications.

Quick Overview for TWEAK antibody (ABIN1176948)

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TWEAK antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: CARL

Product Details anti-TWEAK Antibody

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Human Tweak Transfected Cell Line

Isotype: IgG3 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-TWEAK antibody (ABIN1176948)

Llinàs, Lázaro, de Salort, Matesanz-Isabel, Sintes, Engel: "Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry." in: Immunology letters, Vol. 134, Issue 2, pp. 113-21, (2010) (PubMed).

Xu, Okamoto, Ichikawa, Ando, Tasaka, Masuyama, Ogawa, Yagita, Okumura, Nakao: "TWEAK/Fn14 interaction stimulates human bronchial epithelial cells to produce IL-8 and GM-CSF." in: Biochemical and biophysical research communications, Vol. 318, Issue 2, pp. 422-7, (2004) (PubMed).

Nakayama, Kayagaki, Yamaguchi, Okumura, Yagita: "Involvement of TWEAK in interferon gamma-stimulated monocyte cytotoxicity." in: The Journal of experimental medicine, Vol. 192, Issue 9, pp. 1373-80, (2000) (PubMed).

Lynch, Wang, Lund, Chen, Leal, Wiley: "TWEAK induces angiogenesis and proliferation of endothelial cells." in: The Journal of biological chemistry, Vol. 274, Issue 13, pp. 8455-9, (1999) (PubMed).

Chicheportiche, Bourdon, Xu, Hsu, Scott, Hession, Garcia, Browning: "TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32401-10, (1998) (PubMed).

Target Details for TWEAK

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Alternative Name: TWEAK

Background: The CARL-1 monoclonal antibody specifically binds to TWEAK, also known as TNF-related WEAK inducer of apoptosis. The TWEAK cytokine belongs to the tumor necrosis factor (TNF) ligand family and is encoded by the TNFSF12 (Tumor necrosis factor ligand superfamily member 12) gene. TWEAK is present as a type II transmembrane protein form and a smaller, biologically-active secreted isoform. TWEAK is expressed on most tissues and non-lymphoid tumor cell lines but has weak or no expression on hematopoietic lineage cell lines. TWEAK is not detectable on resting peripheral blood mononuclear cells. However, its expression is upregulated on peripheral blood monocytes in response to IFN-gamma stimulation. TWEAK can act through the TWEAK Receptor (aka, TWEAK-R, TNFRSF12A, Fn14, and CD266) to induce a variety of target cellular responses including NF-kB activation, proliferation, apoptosis, IL-8 and GM-CSF production. Clone CARL-2, a different monoclonal antibody, also binds to TWEAK although it binds to a different TWEAK epitope.
Synonyms: TNFSF12, APO3 ligand, APO3L, DR3LG, TNF-related WEAK inducer of apoptosis

Pathways: Apoptosis