IL-1 beta antibody

Catalog No. ABIN1176987

Product Details anti-IL-1 beta Antibody

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: 50% Neutralization (ND50) at 25 - 100 ng/ml
95% Neutralization at 125 - 500 ng/ml

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse IL-1beta Protein

Clone: B122

Isotype: IgG lambda

Application Details

Application Notes: Neutralization: The NA/LE™ format of this antibody is useful for neutralization of mouse IL-1beta bioactivity. Assay Type: Proliferation at 2 ng/ml mouse IL-1beta (Cat. No. 554577) Indicator cell: TF-1 at 10^5 cells/ml and Preincubation of antibody and ligand = 60 minutes ELISA Capture: The purified B122 antibody (Cat. No. 550605) has been reported to be useful as a capture antibody for a sandwich ELISA for measuring mouse IL-1beta protein levels.
Immunoprecipitation: The B122 antibody has been reported to be useful for the immunoprecipitation of both natural and recombinant forms of mouse IL-1beta protein. Please note that the antibody is not routinely tested for this application at BD Biosciences.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL-1 beta antibody (ABIN1176987)

Fei, Gott, Edwards, Schuyler: "Experimental hypersensitivity pneumonitis: in vitro effects of interleukin-2 and interferon-gamma." in: The Journal of laboratory and clinical medicine, Vol. 126, Issue 5, pp. 485-94, (1995) (PubMed).

Miller, Krasney, Gauvin, Holbrook, Koonz, Abruzzese, Miller, Pagani, Dolle, Ator: "Inhibition of mature IL-1 beta production in murine macrophages and a murine model of inflammation by WIN 67694, an inhibitor of IL-1 beta converting enzyme." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 3, pp. 1331-8, (1995) (PubMed).

Hogquist, Nett, Sheehan, Pendleton, Schreiber, Chaplin: "Generation of monoclonal antibodies to murine IL-1 beta and demonstration of IL-1 in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 5, pp. 1534-40, (1991) (PubMed).

Kitamura, Takaku, Miyajima: "IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1." in: International immunology, Vol. 3, Issue 6, pp. 571-7, (1991) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1beta (IL1B Products)

Synonyms: IL-1 antibody, IL1-BETA antibody, IL1F2 antibody, IL-1BETA antibody, IL1beta antibody, il1-b antibody, zgc:111873 antibody, IL-1B antibody, IL-1beta antibody, Il-1b antibody, IL1B antibody, IL-1 beta antibody, IL-1b antibody, interleukin 1 beta antibody, interleukin 1, beta antibody, IL1B antibody, il1b antibody, Il1b antibody

Background: The B122 antibody recognizes the precursor and mature secreted forms of the mouse IL-1beta protein. B122 neutralizes the biological activity of mouse IL-1beta, but not the biological activities of mouse IL-1alpha nor human IL-1beta. B122 does not recognize mouse IL-2, IL-4, IL-6, IL-10, TNF or IFN-gamma. This antibody also recognizes rat IL-1beta. The immunogen used to generate the B122 hybridoma was a purified, mature form of the mouse IL-1beta protein.

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome