Btk and Itk (pTyr551) antibody (Alexa Fluor 647)

Catalog No. ABIN1177040

The Mouse Monoclonal anti- antibody has been validated for ICS. It is suitable to detect in samples from Human. There is 1 publication available.

Quick Overview for Btk and Itk (pTyr551) antibody (Alexa Fluor 647) (ABIN1177040)

Target: Btk and Itk

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: 24a-BTK

Product Details

Binding Specificity: pTyr551

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Btk Peptide

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References

Wilcox, Berg: "Itk phosphorylation sites are required for functional activity in primary T cells." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37112-21, (2003) (PubMed).

Target Details

Target: Btk and Itk

Background: Bruton's tyrosine kinase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling. The activity of Btk is regulated by Src mediated phosphorylation of the kinase domain at tyrosine 551 (Y551). This event induces Btk kinase activity and subsequent autophosphorylation at Y223. Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate. The Tec family kinase Itk plays a critical role in signal transduction downstream of the T cell antigen receptor and has been implicated in the activation of phospholipase C-gamma1 (PLCgamma1), a key regulator of calcium mobilization and extracellular signal-regulated kinase (ERK) activation. Itk is regulated by an activating transphosphorylation event in which Y511 in the kinase domain is phosphorylated by Lck. The 24a/BTK (Y551) monoclonal antibody recognizes the Y551-phosphorylated form of human Btk and the Y511 phosphorylated form of human Itk.