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EIF4E antibody (pSer209) (PE)

The Mouse Monoclonal anti-EIF4E antibody has been validated for ICS. It is suitable to detect EIF4E in samples from Human, Rat and Mouse. There are 4+ publications available.
Catalog No. ABIN1177052

Quick Overview for EIF4E antibody (pSer209) (PE) (ABIN1177052)

Target

See all EIF4E Antibodies
EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity

  • 125
  • 63
  • 51
  • 19
  • 5
  • 5
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
Human, Rat, Mouse

Host

  • 109
  • 15
  • 2
Mouse

Clonality

  • 99
  • 27
Monoclonal

Conjugate

  • 60
  • 9
  • 8
  • 6
  • 5
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This EIF4E antibody is conjugated to PE

Application

  • 99
  • 52
  • 44
  • 28
  • 27
  • 26
  • 16
  • 16
  • 16
  • 14
  • 7
  • 6
  • 2
  • 1
Intracellular Staining (ICS)

Clone

J77-925
  • Binding Specificity

    • 40
    • 15
    • 8
    • 7
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer209

    Brand

    BD Phosflow™

    Purification

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    Immunogen

    Phosphorylated Human eIF4E Peptide

    Isotype

    IgG1 kappa
  • Application Notes

    Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.

    Sample Volume

    20 μL

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Culjkovic, Topisirovic, Borden: "Controlling gene expression through RNA regulons: the role of the eukaryotic translation initiation factor eIF4E." in: Cell cycle (Georgetown, Tex.), Vol. 6, Issue 1, pp. 65-9, (2007) (PubMed).

    Wendel, Silva, Malina, Mills, Zhu, Ueda, Watanabe-Fukunaga, Fukunaga, Teruya-Feldstein, Pelletier, Lowe: "Dissecting eIF4E action in tumorigenesis." in: Genes & development, Vol. 21, Issue 24, pp. 3232-7, (2007) (PubMed).

    Grolleau, Kaplan, Hanash, Beretta, Richardson: "Impaired translational response and increased protein kinase PKR expression in T cells from lupus patients." in: The Journal of clinical investigation, Vol. 106, Issue 12, pp. 1561-8, (2001) (PubMed).

    Waskiewicz, Flynn, Proud, Cooper: "Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2." in: The EMBO journal, Vol. 16, Issue 8, pp. 1909-20, (1997) (PubMed).

  • Target

    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

    Background

    The eukaryotic translation Initiation Factor 4E (eIF4E) is a 25-kDa phosphoprotein that specifically binds to the 7-methylguanosine-containing cap of mRNA. eIF4E is the rate-limiting component for the initiation of cap-dependent translation by the eIF4F translation initiation complex, which is composed of eIF4E, eIF4G, and eIF4A. This complex promotes the unwinding of secondary structure at the 5' untranslated region of mRNA that is necessary to expose and locate the AUG initiation codon. Other functions of eIF4E have been identified, such as promoting the export of mRNAs that are involved in cell cycle progression from the nucleus and differentially regulating the translation of certain mRNAs in the cytoplasm. Three mechanisms for eIF4E regulation have been identified: Mnk1-mediated phosphorylation on serine 209 (S209) is required for eIF4E binding to the cap structure, over-expression of phosphorylated eIF4E can lead to increased cell proliferation, suppression of apoptosis, and a transformed phenotype, and interactions with nonphosphorylated eIF4E-binding proteins inhibit the formation of the eIF4F complex. The J77-925 monoclonal antibody recognizes the phosphorylated S209 (pS209) of eIF4E.
    Synonyms: eIF-4E, CBP, EIF4E1, EIF4EL1, EIF4F, mRNA cap-binding protein

    Pathways

    BCR Signaling
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