Ezrin antibody (pTyr353) (Alexa Fluor 488)

Catalog No. ABIN1177059

This anti-Ezrin antibody is a Mouse Monoclonal antibody detecting Ezrin in ICS. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Ezrin antibody (pTyr353) (Alexa Fluor 488) (ABIN1177059)

Target: Ezrin (EZR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ezrin antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Clone: I66-386

Product Details anti-Ezrin Antibody (Alexa Fluor 488)

Binding Specificity: pTyr353

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Ezrin Peptide

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-Ezrin antibody (Alexa Fluor 488) (ABIN1177059)

Gautreau, Poullet, Louvard, Arpin: "Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 13, pp. 7300-5, (1999) (PubMed).

Krieg, Hunter: "Identification of the two major epidermal growth factor-induced tyrosine phosphorylation sites in the microvillar core protein ezrin." in: The Journal of biological chemistry, Vol. 267, Issue 27, pp. 19258-65, (1992) (PubMed).

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin

Background: Ezrin is a member of the ERM (ezrin-radixin-moesin) family of proteins that function as crosslinkers between the actin cytoskeleton and the plasma membrane. Ezrin is a substrate for the tyrosine kinase epidermal (EGF) and hepatocyte growth factor (HGF) receptors. Upon EGF or HGF stimulation, ezrin is phosphorylated on tyrosine 353 (Y353), which is required for its interaction with phosphatidylinositol 3 kinase p85 subunit and activation of Akt. The I66-386 monoclonal antibody recognizes the phosphorylated Y353 of human ezrin.

Pathways: Maintenance of Protein Location