Ezrin antibody (pTyr353) (Alexa Fluor 488)
(2 references)-
- Target See all Ezrin (EZR) Antibodies
- Ezrin (EZR)
-
Binding Specificity
- pTyr353
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This Ezrin antibody is conjugated to Alexa Fluor 488
-
Application
- Intracellular Staining (ICS)
- Brand
- BD Phosflow™
- Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Phosphorylated Human Ezrin Peptide
- Clone
- I66-386
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product EZR Primary Antibody
-
anti-Ezrin (EZR) (AA 292-464) antibody
EZR Reactivity: Human WB, ELISA, ICC, FACS Host: Mouse Monoclonal 6F1A9 unconjugated
anti-Ezrin (EZR) (AA 1-295) antibodyEZR Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Ezrin (EZR) (pTyr478) antibodyEZR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Ezrin (EZR) (AA 1-586) antibodyEZR Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
anti-Ezrin (EZR) (pTyr353) antibodyEZR Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Ezrin (EZR) antibodyEZR Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal CPTC-Ezrin-1 unconjugated
anti-Ezrin (EZR) (AA 1-586) antibodyEZR Reactivity: Human WB, ELISA, IHC (p), PLA Host: Mouse Monoclonal 1E11 unconjugated
anti-Ezrin (EZR) (AA 350-470) antibodyEZR Reactivity: Human WB, IP, IF Host: Rabbit Polyclonal unconjugated
anti-Ezrin (EZR) (AA 1-251) antibodyEZR Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Ezrin (EZR) (AA 455-586) antibodyEZR Reactivity: Human, Rat WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
-
- Sample Volume
- 20 μL
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
-
-
: "Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 13, pp. 7300-5, (1999) (PubMed).
: "Identification of the two major epidermal growth factor-induced tyrosine phosphorylation sites in the microvillar core protein ezrin." in: The Journal of biological chemistry, Vol. 267, Issue 27, pp. 19258-65, (1992) (PubMed).
-
: "Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 13, pp. 7300-5, (1999) (PubMed).
-
- Target
- Ezrin (EZR)
- Alternative Name
- Ezrin (EZR Products)
- Background
- Ezrin is a member of the ERM (ezrin-radixin-moesin) family of proteins that function as crosslinkers between the actin cytoskeleton and the plasma membrane. Ezrin is a substrate for the tyrosine kinase epidermal (EGF) and hepatocyte growth factor (HGF) receptors. Upon EGF or HGF stimulation, ezrin is phosphorylated on tyrosine 353 (Y353), which is required for its interaction with phosphatidylinositol 3 kinase p85 subunit and activation of Akt. The I66-386 monoclonal antibody recognizes the phosphorylated Y353 of human ezrin.
- Pathways
- Maintenance of Protein Location
-
