PKA RIIalpha (pSer99) antibody (Alexa Fluor 647)

Catalog No. ABIN1177140

The Alexa Fluor 647-conjugated Mouse Monoclonal anti-PKA RIIalpha antibody (Clone I65-856-286) (ABIN1177140) specifically detects PKA RIIalpha in ICS. The antibody is reactive with Human samples.

Quick Overview for PKA RIIalpha (pSer99) antibody (Alexa Fluor 647) (ABIN1177140)

Target: PKA RIIalpha

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: I65-856-286

Product Details

Binding Specificity: pSer99

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human PKA RIIalpha Peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Target Details

Target: PKA RIIalpha

Alternative Name: PKA RIIa

Background: CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIbeta, RIIalpha, and RIIbeta. These subunits define type I and II PKAs. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and II holoenzymes have three potential C subunits (Calpha, Cbeta, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R subunits, while type I PKA binds Mg/ATP with high affinity. The cAMP-dependent autophosphorylation of the human RIIalpha subunit occurs at Serine 99 (S99) [Entrez Protein Accession #CAA33094]. Most cells express both type I and type II PKAs. Although the Ralpha isoforms are ubiquitously expressed, the Rbeta isoforms are predominantly found in nervous and adipose tissues. In addition to their enzyme regulatory activity, the RIIalpha and RIIbeta subunits determine the subcellular location of the holoenzymes via their interactions with specific intracellular anchoring proteins. The I65-856.286 antibody recognizes human PKA RIIalpha phosphorylated at S99.