Phospholipase C gamma 2 antibody (pTyr759) (Alexa Fluor 647)

Catalog No. ABIN1177150

This Mouse Monoclonal antibody specifically detects Phospholipase C gamma 2 in ICS. It exhibits reactivity toward Human, Rat and Mouseand has been mentioned in 1 publication.

Quick Overview for Phospholipase C gamma 2 antibody (pTyr759) (Alexa Fluor 647) (ABIN1177150)

Target: Phospholipase C gamma 2 (PLCG2)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phospholipase C gamma 2 antibody is conjugated to Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: K86-689-37

Product Details anti-Phospholipase C gamma 2 Antibody (Alexa Fluor 647)

Binding Specificity: pTyr759

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human PLC-gamma2 Peptide

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-Phospholipase C gamma 2 antibody (Alexa Fluor 647) (ABIN1177150)

Holodick, Tumang, Rothstein: "Continual signaling is responsible for constitutive ERK phosphorylation in B-1a cells." in: Molecular immunology, Vol. 46, Issue 15, pp. 3029-36, (2009) (PubMed).

Target Details for Phospholipase C gamma 2

Target: Phospholipase C gamma 2 (PLCG2)

Alternative Name: PLC-gamma 2

Background: The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLC-gamma is the only member that contains SH2 and SH3 domains. These domains enable it to interact with protein tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: PLC-g1, which is ubiquitously expressed, and PLC-g2, which is found primarily in the hematopoietic system. PLC-g2-null mice exhibit impaired B lymphocyte maturation and defects in Fc receptor functions. Phosphorylation of PLC-g2 at tyrosines 753 and 759 (Y759) is required for activation of PLC-g2 enzyme activity. PLC-g2 phosphorylation at Y759 can be induced by stimulation of the B cell receptor in Ramos cells, the collagen receptor in platelets, and the T cell receptor in Jurkat cells, and it occurs downstream of Btk and BLNK in the signaling cascade of activated B cells. The K86-689.37.73 antibody recognizes PLC-g2 phosphorylated at Y759 in the SH2-SH3 linker region.

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling