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PTK2B antibody (pTyr402) (PE)

This Mouse Monoclonal antibody specifically detects PTK2B in ICS. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 6+ publications.
Catalog No. ABIN1177156

Quick Overview for PTK2B antibody (pTyr402) (PE) (ABIN1177156)

Target

See all PTK2B Antibodies
PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Reactivity

  • 111
  • 107
  • 90
  • 8
  • 8
  • 7
  • 6
  • 5
  • 5
  • 5
  • 1
  • 1
  • 1
Human, Mouse, Rat

Host

  • 140
  • 14
  • 3
Mouse

Clonality

  • 136
  • 21
Monoclonal

Conjugate

  • 77
  • 9
  • 7
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
This PTK2B antibody is conjugated to PE

Application

  • 120
  • 53
  • 42
  • 40
  • 39
  • 23
  • 16
  • 12
  • 9
  • 5
  • 1
  • 1
  • 1
Intracellular Staining (ICS)

Clone

L68-1256-272
  • Binding Specificity

    • 24
    • 21
    • 15
    • 10
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr402

    Brand

    BD Phosflow™

    Purification

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    Immunogen

    Phosphorylated Human Pyk2 Peptide

    Isotype

    IgG2b kappa
  • Application Notes

    Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.

    Comment

    BALB/c

    Sample Volume

    20 μL

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Anand, Cucchiarini, Terwilliger, Ganju: "The tyrosine kinase Pyk2 mediates lipopolysaccharide-induced IL-8 expression in human endothelial cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 8, pp. 5636-44, (2008) (PubMed).

    Faure, Corvol, Toutant, Valjent, Hvalby, Jensen, El Messari, Corsi, Kadaré, Girault: "Calcineurin is essential for depolarization-induced nuclear translocation and tyrosine phosphorylation of PYK2 in neurons." in: Journal of cell science, Vol. 120, Issue Pt 17, pp. 3034-44, (2007) (PubMed).

    Orr, Murphy-Ullrich: "Regulation of endothelial cell function BY FAK and PYK2." in: Frontiers in bioscience : a journal and virtual library, Vol. 9, pp. 1254-66, (2004) (PubMed).

    Park, Avraham, Avraham: "RAFTK/Pyk2 activation is mediated by trans-acting autophosphorylation in a Src-independent manner." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33315-22, (2004) (PubMed).

    Gluck: "Acid sensing in renal epithelial cells." in: The Journal of clinical investigation, Vol. 114, Issue 12, pp. 1696-9, (2004) (PubMed).

    Avraham, Park, Schinkmann, Avraham: "RAFTK/Pyk2-mediated cellular signalling." in: Cellular signalling, Vol. 12, Issue 3, pp. 123-33, (2000) (PubMed).

  • Target

    PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

    Alternative Name

    Pyk2

    Background

    Proline-rich tyrosine kinase 2 (Pyk2) is a member of the FAK subfamily of tyrosine protein kinases. Pyk2 responds to a wide variety of inflammatory and stress stimuli, such as cytokines, ligation of integrins or antigen receptors, mechanical strain, and cellular depolarization. A common characteristic of most Pyk2 stimuli is an increase in cytosolic free calcium. Pyk2 is involved in signaling pathways that regulate vital cellular functions in hematopoietic, epithelial, neuronal, endothelial, and other cell types. Activation of Pyk2 initiates its autophosphorylation at tyrosine 402 (Y402), followed by Src-mediated phosphorylation of other Pyk2 tyrosine sites, which enhances its activity and leads to downstream signal transduction through the MAPK and JNK pathways. The L68-1256.272 monoclonal antibody recognizes the phosphorylated Y402 of activated Pyk2.
    Synonyms: Protein tyrosine kinase 2beta, FAK2, FADK 2, CAKbeta, CADTK, RAFTK

    Pathways

    EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling
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