S6 (pSer235) antibody (PE)

Catalog No. ABIN1177163

Product Details

Target: S6

Binding Specificity: pSer235

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: PE

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human ribosomal protein S6 Peptide

Clone: N7-548

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD Phosflow™ permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

References

Blatt, Herrmann, Mirkina, Hadzijusufovic, Peter, Strommer, Hoermann, Mayerhofer, Hoetzenecker, Klepetko, Ghanim, Marth, Füreder, Wacheck, Valenta, Valent: "The PI3-kinase/mTOR-targeting drug NVP-BEZ235 inhibits growth and IgE-dependent activation of human mast cells and basophils." in: PLoS ONE, Vol. 7, Issue 1, pp. e29925, (2012) (PubMed).

van de Laar, van den Bosch, Boonstra, Binda, Buitenhuis, Janssen, Coffer, Woltman: "PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function." in: Blood, Vol. 120, Issue 25, pp. 4982-91, (2012) (PubMed).

Lal, Li, Smith, Sassano, Uddin, Parmar, Tallman, Minucci, Hay, Platanias: "Activation of the p70 S6 kinase by all-trans-retinoic acid in acute promyelocytic leukemia cells." in: Blood, Vol. 105, Issue 4, pp. 1669-77, (2005) (PubMed).

Shah, Hunter: "Critical role of T-loop and H-motif phosphorylation in the regulation of S6 kinase 1 by the tuberous sclerosis complex." in: The Journal of biological chemistry, Vol. 279, Issue 20, pp. 20816-23, (2004) (PubMed).

Shah, Ghosh, Hunter: "Mitotic regulation of ribosomal S6 kinase 1 involves Ser/Thr, Pro phosphorylation of consensus and non-consensus sites by Cdc2." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 16433-42, (2003) (PubMed).

Target Details

Target: S6

Background: Ribosomal protein S6 (~29 kDa calculated and ~32 kDa observed molecular weights) is a component of the 40S ribosomal subunit and belongs to the S6E family of ribosomal proteins. The S6 ribosomal protein plays a role in regulating the translation of RNAs and thus controlling the growth and proliferation of cells. S6 ribosomal protein phosphorylation, especially at multiple C-terminal serine residues S235, S236, S240, and S244, activates S6. The activated S6 ribosomal protein in turn upregulates the ribosomal translation of RNA species coding for other ribosomal proteins, peptide elongation factors and other proteins involved in cell cycle entry and progression. These phosphorylations are mediated by various kinases (e.g., p70S6K and PKCD) activated through cellular responses to growth factors, cytokines, tumor promoting agents, and mitogens. The S6 ribosomal protein can be dephosphorylated in growth-arrested cells. The N7-548 monoclonal antibody specifically detects the S6 ribosomal protein phosphorylated at S235 and S236.
Synonyms: 40S ribosomal protein S6, Phosphoprotein NP33, RPS6, RS6