S6 (pSer235) antibody (Alexa Fluor 488)

Catalog No. ABIN1177165

Product Details

Target: S6

Binding Specificity: pSer235

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Alexa Fluor 488

Application: Intracellular Staining (ICS), BioImaging (BI)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human ribosomal protein S6 Peptide

Clone: N7-548

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD™ Phosflow permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References

Blatt, Herrmann, Mirkina, Hadzijusufovic, Peter, Strommer, Hoermann, Mayerhofer, Hoetzenecker, Klepetko, Ghanim, Marth, Füreder, Wacheck, Valenta, Valent: "The PI3-kinase/mTOR-targeting drug NVP-BEZ235 inhibits growth and IgE-dependent activation of human mast cells and basophils." in: PLoS ONE, Vol. 7, Issue 1, pp. e29925, (2012) (PubMed).

Target Details

Target: S6

Background: Ribosomal protein S6 (~29 kDa calculated and ~32 kDa observed molecular weights) is a component of the 40S ribosomal subunit and belongs to the S6E family of ribosomal proteins. The S6 ribosomal protein plays a role in regulating the translation of RNAs and thus controlling the growth and proliferation of cells. S6 ribosomal protein phosphorylation, especially at multiple C-terminal serine residues S235, S236, S240, and S244, activates S6. The activated S6 ribosomal protein in turn upregulates the ribosomal translation of RNA species coding for other ribosomal proteins, peptide elongation factors and other proteins involved in cell cycle entry and progression. These phosphorylations are mediated by various kinases (e.g., p70S6K and PKCD) activated through cellular responses to growth factors, cytokines, tumor promoting agents, and mitogens. The S6 ribosomal protein can be dephosphorylated in growth-arrested cells. The N7-548 monoclonal antibody specifically detects the S6 ribosomal protein phosphorylated at S235 and S236.
Synonyms: 40S ribosomal protein S6, Phosphoprotein NP33, RPS6, RS6