S6 (pSer240) antibody (Alexa Fluor 647)

Catalog No. ABIN1177167

Product Details

Target: S6

Binding Specificity: pSer240

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Alexa Fluor 647

Application: Intracellular Staining (ICS), BioImaging (BI)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human ribosomal protein S6 Peptide

Clone: N4-41

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD™ Phosflow permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Corradetti, Inoki, Guan: "The stress-inducted proteins RTP801 and RTP801L are negative regulators of the mammalian target of rapamycin pathway." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 9769-72, (2005) (PubMed).

Gu, Santiago, Mitchell: "Synergy between imatinib and mycophenolic acid in inducing apoptosis in cell lines expressing Bcr-Abl." in: Blood, Vol. 105, Issue 8, pp. 3270-7, (2005) (PubMed).

Target Details

Target: S6

Background: Ribosomal protein S6 (~29 kDa calculated and ~32 kDa observed molecular weights) is a component of the 40S ribosomal subunit and belongs to the S6E family of ribosomal proteins. The S6 ribosomal protein plays a role in regulating the translation of RNAs and thus controlling the growth and proliferation of cells. S6 ribosomal protein phosphorylation, especially at multiple C-terminal serine residues S235, S236, S240, and S244, activates S6. The activated S6 ribosomal protein in turn upregulates the ribosomal translation of RNA species coding for other ribosomal proteins, peptide elongation factors and other proteins involved in cell cycle entry and progression. These phosphorylations are mediated by various kinases (e.g., p70S6K and PKCD) activated through cellular responses to growth factors, cytokines, tumor promoting agents, and mitogens. The S6 ribosomal protein can be dephosphorylated in growth-arrested cells. The N4-41 monoclonal antibody specifically detects the S6 ribosomal protein phosphorylated at S240.
Synonyms: 40S ribosomal protein S6, Phosphoprotein NP33, RPS6, RS6