Src antibody (pTyr418) (Alexa Fluor 647)

Catalog No. ABIN1177180

This Mouse Monoclonal antibody specifically detects Src in ICS. It exhibits reactivity toward Human, Mouse, Rat and Chicken and has been mentioned in 3+ publications.

Quick Overview for Src antibody (pTyr418) (Alexa Fluor 647) (ABIN1177180)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Src antibody is conjugated to Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: K98-37

Product Details anti-Src Antibody (Alexa Fluor 647)

Binding Specificity: pTyr418

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Src Peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-Src antibody (Alexa Fluor 647) (ABIN1177180)

Alvarez, Kantarjian, Cortes: "The role of Src in solid and hematologic malignancies: development of new-generation Src inhibitors." in: Cancer, Vol. 107, Issue 8, pp. 1918-29, (2006) (PubMed).

Frame: "Newest findings on the oldest oncogene; how activated src does it." in: Journal of cell science, Vol. 117, Issue Pt 7, pp. 989-98, (2004) (PubMed).

Arias-Salgado, Lizano, Sarkar, Brugge, Ginsberg, Shattil: "Src kinase activation by direct interaction with the integrin beta cytoplasmic domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 23, pp. 13298-302, (2003) (PubMed).

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: Src

Background: The non-receptor protein tyrosine kinases of the Src family have similar domain structures with considerable sequence homologies. They are associated with cell membranes and are activated by a wide variety of cell-surface receptors. Through their participation in cell-signaling cascades that mediate an array of cellular responses, they control essential cellular activities. Src, the prototype of the Src family, is encoded by the SRC protooncogene, which is a homolog of the v-Src gene of the Rous sarcoma virus. Src’s oncogenic potential may be a consequence of its role as a regulator of cell growth, morphology, motility, and adhesion. The protein contains domains that mediate protein-protein interactions and regulation of its tyrosine kinase activity. Mechanisms that regulate Src activity include phosphorylations of serines and tyrosines in most of the domains and interactions with receptor proteins, integrins, and other binding proteins. In particular, autophosphorylation at tyrosine 418 (Y418) activates tyrosine kinase activity. Phosphorylations at other sites are mediated by kinases such as cdc2, CSK, and CHK in signaling cascades and may up- or down-regulate Src activity. The K98-37 monoclonal antibody recognizes the phosphorylated Y418 in the protein kinase domain of activated Src. The orthologous phosphorylation sites in chicken, mouse, and rat Src are Y415, Y423, and Y418, respectively. Because of sequence similarities in this region, it may also recognize the homologous sites of some other Src family members: Lyn (pY396), Fyn (pY420), Hck (pY410), Lck (pY394), and Yes (pY425).
Synonyms: c-Src, p60-Src, SRC, SRC1

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling