IL-10 antibody

Catalog No. ABIN1177290

This anti-IL-10 antibody is a Rat Monoclonal antibody detecting IL-10 in ELISA, Neut and ICS. Suitable for Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for IL-10 antibody (ABIN1177290)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: ELISA, Neutralization (Neut), Intracellular Staining (ICS)

Clone: JES5-16E3

Product Details anti-IL-10 Antibody

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-10. Neutralization activity may be measured with a proliferation assay using 2.0 ng/mL recombinant mouse IL-10 and 50 pg/mL recombinant mouse IL-4 to costimulate D36 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-10 for 60 minutes).
50% Neutralization (ND50) at 2.5 - 20 ng/mL
>95% Neutralization at 30 - 125 ng/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Recombinant mouse IL-10

Isotype: IgG2b

Application Details

Application Notes: ELISA: Biotinylated JES5-16E3 antibody (Cat. No. 554465) is useful as a detection antibody for sandwich ELISA for measuring mouse IL-10 protein levels and can be paired with purified JES5-2A5 antibody as the capture antibody along with recombinant mouse IL-10 (Cat. No. 550070) as the standard. For testing mouse IL-10 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the Mouse IL-10 BD OptEIA™ ELISA Set (Cat. No. 555252).
Flow Cytometry: The JES5-16E3 antibody is useful for flow cytometric analysis to identify and enumerate IL-10 producing cells within mixed cell populations. Direct conjugates of the JES5-16E3 antibody are especially suitable for these experiments, such as PE Rat Anti-Mouse IL-10 (Cat. No. 554467).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL-10 antibody (ABIN1177290)

Litton, Sander, Murphy, OGarra, Abrams: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, (1994) (PubMed).

Schlaak, Schmitt, Hüls, Meyer zum Büschenfelde, Fleischer: "A sensitive and specific bioassay for the detection of human interleukin-10." in: Journal of immunological methods, Vol. 168, Issue 1, pp. 49-54, (1994) (PubMed).

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10

Background: The JES5-16E3 monoclonal antibody specifically binds to the mouse cytokine, Interleukin-10 (IL-10). IL-10 is also known as Cytokine Synthesis Inhibitory Factor (CSIF). It is produced by various activated cell types including CD4+ T cells, CD8+ T cells, NK T cells, B1 B cells, NK cells, macrophages, dendritic cells, mast cells, granulocytes and keratinocytes. IL-10 plays a pivotal role in regulating immune responses and protecting the host from damage caused by inflammatory and autoimmune responses. IL-10 has numerous biological activities including the inhibition of cytokine synthesis by activated T cells, NK cells, monocytes, and macrophages. In the presence of accessory cells, IL-10 inhibits mitogen- or anti-CD3 induced proliferation of T lymphocytes. IL-10 has also been shown to costimulate the development of thymocytes, B cell differentiation and the generation of cytotoxic T cells. The immunogen used to generate the JES5-16E3 hybridoma was recombinant mouse IL-10. JES5-16E3 is a neutralizing antibody.
Synonyms: Interleukin-10, Il10, CSIF, Cytokine synthesis inhibitory factor

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints