Invertase antibody

Catalog No. ABIN117966

The Goat Polyclonal anti-Invertase antibody (ABIN117966) specifically detects Invertase in WB, EIA and IP. The antibody is reactive with Candida sp. samples.

Quick Overview for Invertase antibody (ABIN117966)

Target: Invertase

Reactivity: Candida sp.

Host: Goat

Clonality: Polyclonal

Conjugate: This Invertase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)

Product Details anti-Invertase Antibody

Specificity: This product was prepared from monospecific antiserum. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Invertase [Candida]. Cross reactivity against Invertase from other sources is unknown.

Purification: Delipidation and defibrination.

Immunogen: Invertase [Candida]

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayedagainst 1.0 μg of Invertase [Candida] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 10,000 to 1: 65,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Invertase

Target: Invertase

Background: Invertase hydrolyses the terminal non reducing beta D fructofuranoside residues in beta D fructofuranosides. It is the enzyme that bees use to convert nectar into honey. Industrial and confectionary applications use yeast invertase to split sucrose into fructose and glucose and also to improve the shelf life of food products.Synonyms: Beta-fructofuranosidase, INV1, Saccharase

Gene ID: 2896726

NCBI Accession: XP_451456

UniProt: Q9Y746