Cecropin B antibody
Reactivity: Bombyx mori
IP, WB, EIA
Host: Rabbit
Polyclonal
unconjugated
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- Target See all Cecropin B products
- Cecropin B
- Reactivity
- Bombyx mori
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Cecropin B antibody is un-conjugated
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Application
- Immunoprecipitation (IP), Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using cecropin B coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified cecropin B. Reactivity against other forms of cecropins has not yet been determined.
- Purification
- Immunoaffinity chromatography
- Immunogen
- This whole rabbit serum was prepared by repeated immunizations with a synthetically produced fulllength cecropin B corresponding to a modified sequence from that which is found in silkworm: Lys-Trp-Lys-Val-Phe-Lys-Lys-Ile-Glu-Lys-Val-Gly-Arg-Asn- Ile-Arg-Asn-Gly- Ile-Val-Lys-Ala-Gly-Pro-Ala-Ile-Ala-Val-Leu-Gly-Glu-Ala- Lys-Ala-Leu
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- Application Notes
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Anti-cecropin B is useful for the detection of cecropin B in various immunological assays. This reagent has specific value to study cecropins in transgenic plants. The antibody mayalso recognize naturally occurring cecropin A. Cecropin B has been modified in someinstances by substituting a single amino acid to render the molecule less susceptible toproteolytic cleavage. This reagent is suitable for the detection of cecropin B inImmunoblotting (1: 1000), Immunoprecipitation and ELISA experiments. A single 4400dalton band is detected in protein extracts.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.3 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.01 M Potassium Phosphate, 0.075 M Sodium Chloride, pH 7.2 with 50 % (v/v) Glycerol as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.
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- Target
- Cecropin B
- Alternative Name
- Cecropin-B (Cecropin B Products)
- Synonyms
- CG1878 antibody, Cec antibody, Cec B antibody, Dmel\\CG1878 antibody, cec antibody, cecB antibody, cedcB antibody, crpB antibody, Cecb antibody, CecB antibody, Dsim\\GD17190 antibody, GD17190 antibody, dsim_GLEANR_1734 antibody, Cecropin B antibody, cecropin B antibody, GD17190 gene product from transcript GD17190-RA antibody, CecB antibody, CecB1 antibody, Dsim\CecB antibody
- Background
- Cecropins are produced by insects, particularly under conditions of infection. Cecropins are bioactive peptides that exhibit activities by interacting with membranes and forming transmembrane channels that allow the free flow of electrolytes, metabolites and water across the phospholipid bilayers. Cecropins A, B and D are close homologues consisting of 35-39 residues. They are found in the pupae of the cecropin moth, but related homologues named lepodopteran, bactericidin, moricin and sarcotoxin are produced by other insects.Synonyms: CECB1, Lepidopteran-A/B
- Gene ID
- 693028
- NCBI Accession
- NP_001037460
- UniProt
- P04142
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