PLG antibody

Catalog No. ABIN118044

Cited in 3+ publications. The Goat Polyclonal anti-PLG antibody (ABIN118044) specifically detects PLG in WB, IP and EIA. The antibody is reactive with Human samples.

Quick Overview for PLG antibody (ABIN118044)

Target: PLG (Plasminogen (PLG))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PLG antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-PLG Antibody

Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Plasminogen (Human Plasma). Cross reactivity against Plasminogen from other sources is unknown.

Purification: Delipidation and defibrination

Immunogen: Plasminogen (Human Plasma)

Application Details

Application Notes: Suitable for Immunoblotting (Western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayedagainst 1.0 μg of Plasminogen [Human Plasma] in a standard sandwich ELISA usingPeroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 12,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 With 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -20 °C or below.

References for anti-PLG antibody (ABIN118044)

Funk, Schaarschmidt, Slesiona, Hallström, Horn, Brock: "The glycolytic enzyme enolase represents a plasminogen-binding protein on the surface of a wide variety of medically important fungal species." in: International journal of medical microbiology : IJMM, Vol. 306, Issue 1, pp. 59-68, (2016) (PubMed).

Koenigs, Stahl, Averhoff, Göttig, Wichelhaus, Wallich, Zipfel, Kraiczy: "CipA of Acinetobacter baumannii Is a Novel Plasminogen Binding and Complement Inhibitory Protein." in: The Journal of infectious diseases, Vol. 213, Issue 9, pp. 1388-99, (2016) (PubMed).

Schott, Grosskinsky, Brenner, Kraiczy, Wallich: "Molecular characterization of the interaction of Borrelia parkeri and Borrelia turicatae with human complement regulators." in: Infection and immunity, Vol. 78, Issue 5, pp. 2199-208, (2010) (PubMed).

Target Details for PLG

Target: PLG (Plasminogen (PLG))

Alternative Name: Plasminogen / PLG

Gene ID: 5340

NCBI Accession: NP_000292

UniProt: P00747

Pathways: Complement System, Lipid Metabolism