CD44 antibody

Catalog No. ABIN118198

This Mouse Monoclonal antibody specifically detects CD44 in FACS and IP. It exhibits reactivity toward Mouse.

Quick Overview for CD44 antibody (ABIN118198)

Target: CD44

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 5035-41-1D

Product Details anti-CD44 Antibody

Specificity: Ly24.2 found on more than 90 % bone marrow cells and spleen cells, 56 % of thymus cells and 90 % of lymph node cells. This antibody recognises the following strains of mice:- C57BL/6, C57BL/10, C57/L, C58A, AKR, 129, SJL, NZB, C3H, CE, CBA/H.

Purification: Affinity Chromatography on Protein G

Immunogen: Spleen cells from B6PL-Ly-2a / Ly-3a B6-Ly-1alpha mice. Spleen cells from immunised mice were fused with cells of the mouse P3-NS1-1-Ag4 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1

Gene ID: 12505

NCBI Accession: NP_001034240

UniProt: P15379

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling