CD48 antibody (FITC)

Catalog No. ABIN118219

This Rat Monoclonal antibody specifically detects CD48 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD48 antibody (FITC) (ABIN118219)

Target: CD48

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD48 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: OX-78

Product Details anti-CD48 Antibody (FITC)

Specificity: This antibody labels thymocytes, B and T cells and macrophages. CD48 is a ligand for the mouse CD2 cell surface antigen.

Purification: Affinity chromatography on Protein G

Immunogen: Recombinant soluble mouse CD48. Spleen cells from immunised AO Rats immunised were fused with cells from the Rat Y3 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD48

Target: CD48

Alternative Name: CD48

Background: CD48 is a glycosyl phophatidyl inositol (GPI) anchored cell surface protein also known as Blast1, HuLy m3, BCM1 (in mouse), and OX45 (in rat). CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibrolasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. The CD48 antigen is closely related to the activation antigen, Blast 1.Synonyms: B-lymphocyte activation marker BLAST-1, BCM1, BCM1 surface antigen, BLAST1, Leukocyte antigen MEM-102, MEM-102, TCT.1

Gene ID: 12506

NCBI Accession: NP_031675

UniProt: P18181