ITGA6 antibody (FITC)

Catalog No. ABIN118232

Product Details anti-ITGA6 Antibody (FITC)

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Reactivity: Human, Mouse, Dog, Pig, Monkey, Sheep

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA6 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises CD49F (VLA-6 alpha chain). Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts. VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin.

Purification: Affinity chromatography on Protein G

Immunogen: BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.

Clone: NKI-GoH3

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGA6

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Alternative Name: CD49f / ITGA6 (ITGA6 Products)

Background: CD49f is a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells.Synonyms: CD49 antigen-like family member F, Integrin alpha-6, VLA-6, VLA6

Gene ID: 3655

NCBI Accession: NP_000201

UniProt: P23229

Pathways: CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex