CD90 antibody

Catalog No. ABIN118258

This Mouse Monoclonal antibody specifically detects CD90 in FACS, WB, IP and IHC (fro). It exhibits reactivity toward Mouse, Rat, Rabbit and Guinea Pig.

Quick Overview for CD90 antibody (ABIN118258)

Target: CD90 (THY1)

Reactivity: Mouse, Rat, Rabbit, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD90 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: OX-7

Product Details anti-CD90 Antibody

Specificity: This antibody recognizes Rat and Mouse CD90, also known as Thy1.1. Since Thy1.1 is a monomorphic determinant in Rat but polymorphic in Mice, clone MRC OX-7 reacts with Thy1.1 Mice e.g. AKR and FVB Mice, but not Thy1.2 Mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy. This clone has been reported to induce glomerular nephritis in Wistar Rats.Aff. Const.:The affinity of the Fab' of MRC OX-7 for Rat Thy1 is 3 x 10e9m-1 and for Mouse Thy1.1 is 3 x 10e8m-1 (1)

Purification: Affinity Chromatography on Protein G

Endotoxin Level: Low Endotoxin

Immunogen: Rat Thy1 antigen. Remarks: Spleen cells from an immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/40 - 1/80, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90

Background: Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (Mouse), immature B cells (Rat) and connective tissues.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein

Gene ID: 21838

NCBI Accession: NP_033408

UniProt: P01831

Pathways: Cell-Cell Junction Organization