Sialoadhesin/CD169 antibody

Catalog No. ABIN118292

The Rat Monoclonal anti-Sialoadhesin/CD169 antibody has been validated for FACS and IHC (fro). It is suitable to detect Sialoadhesin/CD169 in samples from Mouse.

Quick Overview for Sialoadhesin/CD169 antibody (ABIN118292)

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sialoadhesin/CD169 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MOMA-1

Product Details anti-Sialoadhesin/CD169 Antibody

Specificity: Clone MOMA-1 is a useful marker for the identification of Macrophage subpopulations in various organs, mostly characterised by a high level of non-specific esterase expression. The staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. The marker is also very suitable for differentiation of non-metallophilic marginal zone Macrophages as detected by ER-TR9 (Cat.-No BM4011). In addition , MOMA-1 detects Macrophages at inflammatory sites and is positive with Kupffer cells. The antigen is differentially induced in in vitro derived Macrophages depending on the colony-stimulating factor applied (IL-3 > M-CSF > GM-CSF). The antigen detected by MOMA-1 is CD169, also known as Sialoadhesin. Antigen Distribution Isolated Cells: No reactivity of MOMA-1 was found with dendritic cells, peritoneal resident Macrophages, peritoneal exudate cells, bone marrow or blood cells. Tissue Sections: Distinct Macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer's patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1. No MOMA-1-positive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a Macrophage subpopulation in the lamina propria of the villi of the small intestine.Antigen Distribution

Cross-Reactivity (Details): Species reactivity (tested):Mouse: Subpopulation of Mature Resident Tissue Macrophages.

Purification: Affinity Chromatography.

Immunogen: Mouse lymph node tissue. Remarks: The antigen is found intracellularly and on cell surface on Metallophilic Macrophages.

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on frozen sections: 2 μg/mL (1/200). Fixation: Acetone, Positive control: Mouse spleen. Flow cytometry. Does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water (= 0.4 mg/mL Stock Solution).

Concentration: 0.4 mg/mL

Buffer: Stock solutions contains PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20 °C upon receipt.

Target Details for Sialoadhesin/CD169

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Alternative Name: CD169 / SIGLEC1

Background: Metallophilic macrophages are a subpopulation of mature resident tissue macrophages. They show high non specific esterase activity and can be distinguished from splenic marginal zone macrophages by antibody staining and the lack of FITC-Ficoll uptake.Synonyms: Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec-1

Gene ID: 20612

NCBI Accession: NP_035556

UniProt: Q62230