The Mouse Monoclonal anti-MHC Class II I-Ab antibody (Clone 25-5-16S) (ABIN118320) specifically detects MHC Class II I-Ab in FACS and CyTox.
The antibody is reactive with Mouse samples.
Flow cytometry: see protocol. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.2 mg/mL
Buffer
PBS and 0.09 % NaN3
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage
4 °C/-20 °C
Storage Comment
Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
MHC Class II I-Ab
Background
Class II antigens are most highly expressed on antigen-presenting cells including B cells, macrophages, dendritic cells and certain epithelial cells.Synonyms: H-2 class II histocompatibility antigen I-A beta chain, H2-Eb1