CD5 antibody (FITC)

Catalog No. ABIN118495

This Mouse Monoclonal antibody specifically detects CD5 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD5 antibody (FITC) (ABIN118495)

Target: CD5

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MF7-14-5

Product Details anti-CD5 Antibody (FITC)

Specificity: This antibody recognises the CD5 cell surface glycoprotein, a 67 kDa antigen expressed by T lymphocytes and by a subset of B lymphocytes.

Purification: Affinity chromatography on Protein G

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells or 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: CD5 is a 55 kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5

Gene ID: 921

NCBI Accession: NP_055022

UniProt: P06127