CD9 antibody

Catalog No. ABIN118502

The Mouse Monoclonal anti-CD9 antibody is suitable to detect CD9 in samples from Human, Cow, Dog, Horse, Rabbit and Cat. It has been validated for FACS, WB and IHC (fro).

Quick Overview for CD9 antibody (ABIN118502)

Target: CD9

Reactivity: Human, Cow, Dog, Horse, Rabbit, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MM2-57

Product Details anti-CD9 Antibody

Cross-Reactivity (Details): Species reactivity (expected):Mustelid.
Species reactivity (tested):Human, Horse, Bovine, Rabbit, Rhesus Monkey, Dog, Cat, Ferret, Llama, Mink and Pig.

Purification: Affinity Chromatography on Protein G.

Immunogen: Human platelet membrane. Spleen cells from BALB/c mice immunised were fused with cells of the mouse SP2/0 myeloma line.

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: 1/100-1/200, Use 10 μL to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on frozen sections: 1/500-1/1000. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD9

Target: CD9

Alternative Name: CD9

Background: CD9 is a member of the tetraspan gene family. CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve.Synonyms: 5H9 antigen, Cell growth-inhibiting gene 2 protein, GIG2, Leukocyte antigen MIC3, MIC3, Motility-related protein, TSPAN29, Tetraspanin-29, p24

Gene ID: 928

NCBI Accession: NP_001760

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization