CD13 antibody

Catalog No. ABIN118526

Product Details anti-CD13 Antibody

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD13 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Functional Studies (Func), Enzyme Immunoassay (EIA)

Specificity: The antibody recognises the Human CD13 cell surface glycoprotein, a 150 kDa molecule expressed by granulocytes and monocytes, and by myeloid leukaemia cells.

Purification: Affinity Chromatography on Protein G

Immunogen: Human AML cells.

Clone: WM15

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. Immunohistochemistry (frozen sections). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Clone WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 Molecule immunoprecipitates.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4 containing 15 mM Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD13 antibody (ABIN118526)

Takeda, Marumo, Nara, Feng, Asao, Wakabayashi: "Selective induction of anti-inflammatory monocyte-platelet aggregates in a model of pulsatile blood flow at low shear rates." in: Platelets, Vol. 27, Issue 6, pp. 583-92, (2017) (PubMed).

McCormack, Muji?, Osdal, Bruserud, Gjertsen: "Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia." in: Blood, Vol. 121, Issue 7, pp. e34-42, (2013) (PubMed).

Wenzel, Zabojszcza, Carl, Taubert, Lass, Harris, Ho, Schulz, Hummel, Hubner, Osterziel, Spuler: "Increased susceptibility to complement attack due to down-regulation of decay-accelerating factor/CD55 in dysferlin-deficient muscular dystrophy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 9, pp. 6219-25, (2005) (PubMed).

Lau, Mollnau, Eiserich, Freeman, Daiber, Gehling, Brümmer, Rudolph, Münzel, Heitzer, Meinertz, Baldus: "Myeloperoxidase mediates neutrophil activation by association with CD11b/CD18 integrins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 2, pp. 431-6, (2005) (PubMed).

Sun, Woodcock, Rapoport, Stomski, Korpelainen, Bagley, Goodall, Smith, Gamble, Vadas, Lopez: "Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist." in: Blood, Vol. 87, Issue 1, pp. 83-92, (1996) (PubMed).

Favaloro, Browning, Facey: "CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated." in: Experimental hematology, Vol. 21, Issue 13, pp. 1695-701, (1993) (PubMed).

Bradstock, Favaloro, Kabral, Kerr, Hughes, Musgrove: "Myeloid progenitor surface antigen identified by monoclonal antibody." in: British journal of haematology, Vol. 61, Issue 1, pp. 11-20, (1985) (PubMed).

Target Details for CD13

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Alternative Name: CD13 (ANPEP Products)

Background: CD13 is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas. CD13 functions as an aminopeptidase enzyme and is also a receptor for coronavirus.Synonyms: ANPEP, APN, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, PEPN, gp150

Gene ID: 290

NCBI Accession: NP_001141

UniProt: P15144

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones