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CD33 antibody (FITC)

This anti-CD33 antibody is a Mouse Monoclonal antibody detecting CD33 in FACS. Suitable for Human.
Catalog No. ABIN118635

Quick Overview for CD33 antibody (FITC) (ABIN118635)

Target

See all CD33 Antibodies
CD33

Reactivity

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Human

Host

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Mouse

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Monoclonal

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This CD33 antibody is conjugated to FITC

Application

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Flow Cytometry (FACS)

Clone

WM53
  • Specificity

    This antibody recognises the CD33 cell surface glycoprotein.

    Purification

    Affinity chromatography on Protein G

    Immunogen

    Human AML cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.

    Isotype

    IgG1
  • Application Notes

    Flow cytometry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target

    CD33

    Alternative Name

    CD33 / SIGLEC3

    Background

    CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67 kDa M.W. surface antigen. CD33 is a 67kD molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67

    Gene ID

    945

    NCBI Accession

    NP_001763

    UniProt

    P20138
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