CD44 antibody

Catalog No. ABIN118668

This anti-CD44 antibody is a Mouse Monoclonal antibody detecting CD44 in FACS, WB, IHC (p) and IHC (fro). Suitable for Human.

Quick Overview for CD44 antibody (ABIN118668)

Target: CD44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: VFF-8

Product Details anti-CD44 Antibody

Specificity: SM1113P specifically recognises an epitope encoded by exon v5 on the variant portion of human CD44.

Purification: Purified

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: dilute 1:10, use 10 μL to label 10^6 cells in 100 μL. Immunohistochemistry: frozen sections and paraffin embedded sections: 1:10. This product requires antigen retrieval using heat treatment prior to staining of Paraffin sections. Sodium Citrate buffer pH 6.0 is recommended for this purpose. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: Synonyms: CD44, CDw44, ECMR-III, Epican, Extracellular matrix receptor-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein I

Gene ID: 960

NCBI Accession: NP_000601

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling