CD44 antibody

Catalog No. ABIN118703

The Mouse Monoclonal anti-CD44 antibody has been validated for FACS, WB, IHC (p) and IHC (fro). It is suitable to detect CD44 in samples from Human.

Quick Overview for CD44 antibody (ABIN118703)

Target: CD44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: VFF-17

Product Details anti-CD44 Antibody

Purification: Purified

Immunogen: Human CD44v7/8.

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: 1:10 - 1:100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: frozen sections 1:100 - 1:1000. Immunohistochemistry on paraffin sections: 1:100, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: Synonyms: CD44, CDw44, ECMR-III, Epican, Extracellular matrix receptor-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein I

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling