ITGA5 antibody

Catalog No. ABIN118715

The Mouse Monoclonal anti-ITGA5 antibody has been validated for FACS, IP and IHC (fro). It is suitable to detect ITGA5 in samples from Human and Dog.

Quick Overview for ITGA5 antibody (ABIN118715)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: JBS5

Product Details anti-ITGA5 Antibody

Specificity: This antibody reacts with the Alpha 5 integrin. Has been shown to stimulate homotypic cellular aggregation of alpha 5 beta 1 positive lymphoid cell lines. It also inhibits the adherence of cells to fibronectin.

Purification: Affinity Chromatography on Protein G

Immunogen: Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation: 1-5 μg/mL. Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 1x10^6 cells in 100 μL of whole blood. Immunohistochemistry on Frozen Sections: 0.5-1 μg/mL. Recommended Positive Control: Human Endothelium. Functional assays: Removal of Sodium Azide is recommended prior to use.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: CD49e / ITGA5

Background: CD49e is the integrin alpha 5 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor that is known variously: in lymphocytes as very late (activation) antigen 5 (VLA5), in platelets as glycoprotein IcIIa, and in fibroblasts as extracellular matrix receptor 6 (ECMVI). In addition to adhesion, integrins are known to participate in cell surface mediated signalling.Synonyms: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5

Gene ID: 3678

NCBI Accession: NP_002196

UniProt: P08648

Pathways: Integrin Complex