CD55 antibody

Catalog No. ABIN118738

This Mouse Monoclonal antibody specifically detects CD55 in FACS and IHC (fro). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for CD55 antibody (ABIN118738)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 67

Product Details anti-CD55 Antibody

Purification: Affinity Chromatography on Protein G

Immunogen: K562 cells

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: use 10 μL of 1/10-1/25 diluted antibody to label 10^6 cells in 100 μL. We do not recommend the use of this reagent to stain erythrocytes. Immunohistochemistry on Frozen Sections (1/100-1/1000). Recommended positive control: Human Tonsil. (Successful use in Immunohistochemistry on Paraffin embedded sections (1/50-1/100)using heat treatment for antigen retrieval) was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD55 antibody (ABIN118738)

Wenzel, Zabojszcza, Carl, Taubert, Lass, Harris, Ho, Schulz, Hummel, Hubner, Osterziel, Spuler: "Increased susceptibility to complement attack due to down-regulation of decay-accelerating factor/CD55 in dysferlin-deficient muscular dystrophy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 9, pp. 6219-25, (2005) (PubMed).

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55 / DAF

Background: Avoidance by host tissues of attack by autologous complement proteins is dependent in part on the activities of membrane regulatory factors. One molecule involved in this control is a 70 kD glycoprotein termed decay-accelerating factor (DAF), or CD55. Interruption by CD55 of the complement sequence at an early step in activation effectively halts progression of the cascade and prevents consequent cell injury. In man, CD55 is expressed on the plasma membrane of all cell types that are in intimate contact with plasma complement proteins. This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. CD55 also acts as the receptor for echovirus 7 and related viruses(echoviruses 13, 21, 29 and 33). It is attached to the membrane by a GPI-anchor.Synonyms: Complement decay-accelerating factor

Gene ID: 9606

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity