CD59 antibody

Catalog No. ABIN118751

The Mouse Monoclonal anti-CD59 antibody has been validated for WB, FACS, IHC (p), Func and IHC (fro). It is suitable to detect CD59 in samples from Human.

Quick Overview for CD59 antibody (ABIN118751)

Target: CD59

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD59 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MEM-43

Product Details anti-CD59 Antibody

Specificity: Reacts with a PI-linked glycoprotein, Mw 18-20 kDa found on all types of leucocytes including platelets. The antigen is expressed on many haemapoietic and non-haemapoietic cells. MEM-43 has been shown to stimulate NK activity. CD59 is expressed by erythrocytes and therefore staining in whole blood systems requires a high concentration of antibody. The epitope recognised by MEM-43 is lost after reduction, therefore, non-reducing conditions are required for western blotting techniques (2, 5).

Purification: Affinity Chromatography on Protein A.

Isotype: IgG2a

Application Details

Application Notes: ELISA. Immunoprecipitation. Western blot (non-reducing conditions). Flow Cytometry (Use 10 μL of 1/5-1/10 diluted antibody to label 106 cells in 100 μL). Immunohistochemistry on Frozen and Paraffin Sections: Requires antigen retrieval using heat treatment methods prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Positive control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD59

Target: CD59

Alternative Name: CD59

Background: CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. It is a potent inhibitor of the complement membrane attack complex action. It acts by binding to the C8 and/or C9 complements of the membrane attack complex, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is also involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin

Gene ID: 966

NCBI Accession: NP_000602

UniProt: P13987

Pathways: Complement System