CEACAM8 antibody

Catalog No. ABIN118779

The Mouse Monoclonal anti-CEACAM8 antibody (Clone 80H3) (ABIN118779) specifically detects CEACAM8 in FACS and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for CEACAM8 antibody (ABIN118779)

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 80H3

Product Details anti-CEACAM8 Antibody

Specificity: SM1154 recognises the CGM6 gene product of the carcinoembryonic gene family.

Purification: Affinity Chromatography on Protein A.

Immunogen: Human leucocytes from individuals with CML

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 5x10e5 cells or 100 μL whole blood. Immunohistochemistry on Frozen Sections (1/25-1/100). Recommended positive control tissue: Bone Marrow. Does not work on Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL IgG

Buffer: PBS, 0.09 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CEACAM8

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Alternative Name: CD66b / CEACAM8

Background: CD66b is a 95-100 kD, GPI anchored molecule. CD66b is expressed strongly by mature granulocytes and meta-myelocytes, but only weakly by bone marrow myelocytes. Peripheral blood monocytes and lymphocytes do not express significant amounts of this antigen. The antibody reacts with cells from CML in the chronic phase. The CD67 cluster was re-designated as CD66b at the Vth Leucocyte Typing Workshop in Boston, 1993.Synonyms: CD67, CGM6, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, NCA-95, Non-specific cross-reacting antigen NCA-95

Gene ID: 1088

NCBI Accession: NP_001807

UniProt: P31997