Integrin beta 4 antibody

Catalog No. ABIN118870

This Mouse Monoclonal antibody specifically detects Integrin beta 4 in WB, FACS, IP and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Integrin beta 4 antibody (ABIN118870)

Target: Integrin beta 4 (ITGB4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 450-9D

Product Details anti-Integrin beta 4 Antibody

Specificity: This antibody recognises the beta4 integrin (also known as CD104). This antibody recognises an extracellular epitope on the CD104 Molecule.

Purification: Affinity chromatography on Protein G

Immunogen: Purified alpha6 beta4 integrin from A431 cells Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Western Blot. Flow cytometry: 1:25 - 1:100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections: 1:500 - 1:1000. Recommended positive control tissue: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Normal saline

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C only. This product should be stored undiluted.

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: CD104 / Integrin beta-4

Background: CD104 is a 205kD glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumour cell lines.Synonyms: GP150, ITGB4

Gene ID: 3691

NCBI Accession: NP_000204

UniProt: P16144

Pathways: Integrin Complex