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Integrin beta 4 antibody (FITC)

The Mouse Monoclonal anti-Integrin beta 4 antibody has been validated for FACS. It is suitable to detect Integrin beta 4 in samples from Human.
Catalog No. ABIN118871

Quick Overview for Integrin beta 4 antibody (FITC) (ABIN118871)

Target

See all Integrin beta 4 (ITGB4) Antibodies
Integrin beta 4 (ITGB4)

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Integrin beta 4 antibody is conjugated to FITC

Application

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Flow Cytometry (FACS)

Clone

450-9D
  • Specificity

    This antibody recognises the b4 integrin (also known as CD104). It recognises an extracellular epitope on the CD104 Molecule.

    Purification

    Affinity chromatography on Protein G.

    Immunogen

    Purified alpha6beta4 integrin from A431 cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

    Isotype

    IgG1
  • Application Notes

    Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.1 mg/mL

    Buffer

    PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target

    Integrin beta 4 (ITGB4)

    Alternative Name

    CD104 / Integrin beta-4

    Background

    CD104 is a 205kD glycoprotein which associates with the a6 integrin to form the a6/b4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumour cell lines.Synonyms: GP150, ITGB4

    Gene ID

    3691

    NCBI Accession

    NP_000204

    UniProt

    P16144

    Pathways

    Integrin Complex
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