TPSAB1 antibody

Catalog No. ABIN118895

The Mouse Monoclonal anti-TPSAB1 antibody has been validated for WB, IF, IHC (p), IHC (fro) and EIA. It is suitable to detect TPSAB1 in samples from Human, Rat, Dog, Cat and Monkey.

Quick Overview for TPSAB1 antibody (ABIN118895)

Target: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Reactivity: Human, Rat, Dog, Cat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This TPSAB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: AA1

Product Details anti-TPSAB1 Antibody

Specificity: This antiobdy recognises Mast Cell Tryptase, both alpha and beta isoforms. This antibody is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology.

Purification: Affinity Chromatography on Protein G

Immunogen: Human mast cell tryptase purified from Human lung tissue. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/10,000): This product requires either protein digestion pre-treatment of paraffin sections e.g. pronase, or HIER, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TPSAB1

Target: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Alternative Name: Tryptase beta-1 (TPSAB1)

Background: Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.Synonyms: Mast Cell Tryptase, TPSB1, Tryptase I

Gene ID: 100507804

NCBI Accession: NP_003285

UniProt: Q15661