TNNT3 antibody

Catalog No. ABIN118971

This anti-TNNT3 antibody is a Mouse Monoclonal antibody detecting TNNT3 in WB, IHC (p), EIA and IHC (fro). Suitable for Human and Chicken.

Quick Overview for TNNT3 antibody (ABIN118971)

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Reactivity: Human, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNNT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: T1-61

Product Details anti-TNNT3 Antibody

Purification: Affinity Chromatography on Protein G

Immunogen: Troponin T, purified from chicken breast muscle. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot. Immunohistochemistry on Frozen and Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control Tissue: Skeletal muscle.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TNNT3

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Alternative Name: Fast Skeletal Muscle Troponin T

Background: The contraction of striated muscle is initiated by the binding of intracellular calcium to the troponin complex located in the thin filaments. The complex consists of the 3 different troponins, troponin C, troponin T, and troponin I. Troponin T is the tropomyosin binding subunit of troponin which confers calcium sensitivity to striated muscle actomyosin ATPase activity. TNNT3 (Troponin T type 3 (skeletal, fast)) is one of 3 identified troponin T proteins which are encoded by different genes.Synonyms: Beta TnTF, TNNT3, TnTf

Gene ID: 9031

UniProt: P12620