Ubiquitin antibody

Catalog No. ABIN118999

This Mouse Monoclonal antibody specifically detects Ubiquitin in WB, IHC (p), IHC (fro) and EIA. It exhibits reactivity toward Human and Cow.

Quick Overview for Ubiquitin antibody (ABIN118999)

Target: Ubiquitin (Ub)

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 1B4-UB

Product Details anti-Ubiquitin Antibody

Specificity: This antibody recognises ubiquitin.

Purification: Affinity chromatography on Protein G

Immunogen: Ubiquitin from bovine erythrocytes conjugated to bovine gamma Globulin. Spleen cells from immunised BALB/c mouse were fused with cells of the mouse PAI myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot. Flow Cytometry: 1/10 - 1/50, Membrane permeabilisation is required for this application. Immunohistochemistry on frozen sections: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/500 - 1/1000, This product does not require protein digestion pre-treatment of paraffin sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Human hypophysis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Synonyms: Polyubiquitin-B, UBB

Gene ID: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway