Synaptophysin antibody

Catalog No. ABIN119030

The Mouse Monoclonal anti-Synaptophysin antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect Synaptophysin in samples from Human, Rat, Pig and Hamster.

Quick Overview for Synaptophysin antibody (ABIN119030)

Target: Synaptophysin (SYP)

Reactivity: Human, Rat, Pig, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Synaptophysin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: SP15

Product Details anti-Synaptophysin Antibody

Specificity: This antibody recognises an antigen which appears to be identical to the synaptic vesicle protein synaptophysin. It reacts with synaptophysin expressed in CHO cells (3., 8.).

Purification: Gel filtration.

Immunogen: Crude human synaptophysin immunoprecipitate. Spleen cells from immunised BALB/c mice were fused with cells of the NSO mouse myeloma cell line.

Isotype: IgM

Application Details

Application Notes: ELISA. Western Blot: 1/500. Immunohistochemistry on Paraffin Sections: 1/25. Recommended Positive Control: Pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Synaptophysin

Target: Synaptophysin (SYP)

Alternative Name: Synaptophysin / SYP

Background: Synonyms: Major synaptic vesicle protein p38, Neuroendocrine Marker

Gene ID: 6855

NCBI Accession: NP_003170

UniProt: P08247

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity