FITC antibody

Catalog No. ABIN119046

The Rat Monoclonal anti-FITC antibody (Clone LO-FLUO-1) (ABIN119046) specifically detects FITC in IHC (fro) and FACS.

Quick Overview for FITC antibody (ABIN119046)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Host: Rat

Clonality: Monoclonal

Conjugate: This FITC antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Clone: LO-FLUO-1

Product Details anti-FITC Antibody

Purification: Affinity chromatography

Immunogen: FITC conjugated mouse immunoglobulin. Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line.

Isotype: IgM

Application Details

Application Notes: Flow cytometry. ELISA: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC

Target Type: Chemical

Background: FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.Synonyms: Fluorescein Isothiocyanate